anti jarid2  (Novus Biologicals)

Journal: BMC Cancer

Article Title: JARID2, a novel regulatory factor, promotes cell proliferation, migration, and invasion in oral squamous cell carcinoma

doi: 10.1186/s12885-024-12457-6

Figure Lengend Snippet: ( A ) TCGA transcriptome data showed that JARID2 had a significantly higher expression in OSCC samples than in normal samples. ( B ) Immunohistochemical staining showed that JARID2 was expressed in adjacent normal oral mucosa ( n = 35) and OSCC samples ( n = 35); Using human brain tissue as a positive control. Use non immune IgG instead of primary antibody as negative control (left: 100 x, right: 200 x). ( C ) Overall survival in patients with OSCC according to JARID2 protein expression. ( D ) JARID2 expression was higher in normal tissue adjacent to cancer in patients than in cancer tissue. ( E ) RT-qPCR showed that the expression of JARID2 was significantly lower in adjacent normal oral mucosa ( n = 20) than in OSCC samples ( n = 20). ( F ) Expression of JARID2 in HACAT, SCC25, and HN30 cells. ( G ) Evaluation of knockout and overexpression efficiency by RT-qPCR. * p < 0.05, **** p < 0.0001

Article Snippet: These sections were baked at 65 °C for 2 h, dewaxed, hydrated, and underwent heat treatment at 100 °C in a citrate buffer solution (pH = 6.0) for 15 min. After incubating with 3% hydrogen peroxide for 10 min, the sections were then incubated overnight at 4 °C with an antibody against JARID2 (GeneTex, USA, GTX129019, 1:500).

Techniques: Expressing, Immunohistochemical staining, Staining, Positive Control, Negative Control, Quantitative RT-PCR, Knock-Out, Over Expression

Journal: BMC Cancer

Article Title: JARID2, a novel regulatory factor, promotes cell proliferation, migration, and invasion in oral squamous cell carcinoma

doi: 10.1186/s12885-024-12457-6

Figure Lengend Snippet: ( A ) Heatmap of the top 20 co-expressed genes of JARID2. ( B ) The co-expression network of JARID2

Article Snippet: These sections were baked at 65 °C for 2 h, dewaxed, hydrated, and underwent heat treatment at 100 °C in a citrate buffer solution (pH = 6.0) for 15 min. After incubating with 3% hydrogen peroxide for 10 min, the sections were then incubated overnight at 4 °C with an antibody against JARID2 (GeneTex, USA, GTX129019, 1:500).

Techniques: Expressing

Journal: BMC Cancer

Article Title: JARID2, a novel regulatory factor, promotes cell proliferation, migration, and invasion in oral squamous cell carcinoma

doi: 10.1186/s12885-024-12457-6

Figure Lengend Snippet: ( A ) Functional enrichment analysis based on GSEA between high and low JARID2 expression groups. ( B ) Functional enrichment analysis based on GSVA between high and low JARID2 expression groups. ( C ) The correlation of JARID2 expression with 28 immune cell types. ( D ) Waterfall plots showing the differences in gene mutation between high and low expression groups

Article Snippet: These sections were baked at 65 °C for 2 h, dewaxed, hydrated, and underwent heat treatment at 100 °C in a citrate buffer solution (pH = 6.0) for 15 min. After incubating with 3% hydrogen peroxide for 10 min, the sections were then incubated overnight at 4 °C with an antibody against JARID2 (GeneTex, USA, GTX129019, 1:500).

Techniques: Functional Assay, Expressing, Mutagenesis

Journal: BMC Cancer

Article Title: JARID2, a novel regulatory factor, promotes cell proliferation, migration, and invasion in oral squamous cell carcinoma

doi: 10.1186/s12885-024-12457-6

Figure Lengend Snippet: ( A ) The correlation between drug sensitivity and JARID2 expression. ( B ) The difference in drug sensitivity between high and low JARID2 expression group

Article Snippet: These sections were baked at 65 °C for 2 h, dewaxed, hydrated, and underwent heat treatment at 100 °C in a citrate buffer solution (pH = 6.0) for 15 min. After incubating with 3% hydrogen peroxide for 10 min, the sections were then incubated overnight at 4 °C with an antibody against JARID2 (GeneTex, USA, GTX129019, 1:500).

Techniques: Expressing

Journal: BMC Cancer

Article Title: JARID2, a novel regulatory factor, promotes cell proliferation, migration, and invasion in oral squamous cell carcinoma

doi: 10.1186/s12885-024-12457-6

Figure Lengend Snippet: ( A ) The CCK-8 method was used to detect the proliferation of JARID2 knockdown SCC25 and HN30 cells compared to the nc group. ( B ) Migration ability of JARID2 knockdown SCC25 and HN30 cells compared to the nc group by wound healing test. ( C ) Transwell assays were performed to indicate the invasion of JARID2 knockdown SCC25 and HN30 cells compared to the nc group. ns: not significant; ** p < 0.01, *** p < 0.001, and **** p < 0.0001

Article Snippet: These sections were baked at 65 °C for 2 h, dewaxed, hydrated, and underwent heat treatment at 100 °C in a citrate buffer solution (pH = 6.0) for 15 min. After incubating with 3% hydrogen peroxide for 10 min, the sections were then incubated overnight at 4 °C with an antibody against JARID2 (GeneTex, USA, GTX129019, 1:500).

Techniques: CCK-8 Assay, Knockdown, Migration

Journal: BMC Cancer

Article Title: JARID2, a novel regulatory factor, promotes cell proliferation, migration, and invasion in oral squamous cell carcinoma

doi: 10.1186/s12885-024-12457-6

Figure Lengend Snippet: The relationship between JARID2 expression and clinicopathological factors in patients with OSCC

Article Snippet: These sections were baked at 65 °C for 2 h, dewaxed, hydrated, and underwent heat treatment at 100 °C in a citrate buffer solution (pH = 6.0) for 15 min. After incubating with 3% hydrogen peroxide for 10 min, the sections were then incubated overnight at 4 °C with an antibody against JARID2 (GeneTex, USA, GTX129019, 1:500).

Techniques: Expressing